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goat anti rabbit secondary antibody af647  (SouthernBiotech)


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    Structured Review

    SouthernBiotech goat anti rabbit secondary antibody af647
    Goat Anti Rabbit Secondary Antibody Af647, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+rabbit+secondary+antibody+af647/Goat+Anti-Rabbit+IgG(H%2BL)%2C+Mouse%2FHuman+ads-Alexa+Fluor+647/pmc12995837-54-71-76
    Average 93 stars, based on 11 article reviews
    goat anti rabbit secondary antibody af647 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Sudden death-associated KCNH2 variants exert opposing effects on a nuclear subdomain of the cardiac potassium channel hERG1.
    Article Snippet: We washed the fixed cells 3 times using PBS (Gibco, Cat. No. 10010023) and stained cell nuclei using 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher, Cat. No. 62248) for 15 minutes. .. For un-transfected cells, prior to DAPI staining, we immunolabeled with a 1:150 dilution of pan-hERG primary antibody (Enzo Life Sciences, Cat. No. ALX-215049-R100) and a 1:250 dilution of goat anti-rabbit secondary antibody AF647 (Southern Biotech, Cat. No. 4050-31) to stain for the hERG1a C-terminal domain. .. We then washed 3 more times with PBS (Gibco, Cat. No. 10010023) and mounted the coverslips Jo urn al Pr -pr oo f 9 on microscope slides using ProLongTM Gold antifade reagent (Thermo Fisher, Cat. No. P36930).

    Article Title: Sudden Death-Associated KCNH2 Variants Have Opposing Effects on hERG1 NP Function
    Article Snippet: We washed the fixed cells 3 times using PBS (Gibco, Cat. No. 10010023) and stained cell nuclei using 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher, Cat. No. 62248) for 15 minutes. .. For un-transfected cells, prior to DAPI staining, we immunolabeled with a 1:150 dilution of pan-hERG primary antibody (Enzo Life Sciences, Cat. No. ALX-215-049-R100) and a 1:250 dilution of goat anti-rabbit secondary antibody AF647 (Southern Biotech, Cat. No. 4050-31) to stain for the hERG1a C-terminal domain. .. We then washed 3 more times with PBS (Gibco, Cat. No. 10010023) and mounted the coverslips on microscope slides using ProLongTM Gold antifade reagent (Thermo Fisher, Cat. No. P36930).

    Article Title: Sudden death–associated KCNH2 variants exert opposing effects on a nuclear subdomain of the cardiac potassium channel hERG1
    Article Snippet: .. After 48 h, we fixed cells with 4% paraformaldehyde in PBS for 15 min. We washed the fixed cells three times using PBS (Gibco, catalog no.: 10010023) and stained cell nuclei using 1 μg/ml 4′,6-diamidino-2-phenylindole (Thermo Fisher, catalog no.: 62248) for 15 min. For untransfected cells, prior to 4′,6-diamidino-2-phenylindole staining, we immunolabeled with a 1:150 dilution of pan-hERG primary antibody (Enzo Life Sciences, catalog no.: ALX-215-049-R100) and a 1:250 dilution of goat anti-rabbit secondary antibody AF647 (Southern Biotech, catalog no.: 4050-31) to stain for the hERG1a C-terminal domain. .. We then washed three more times with PBS (Gibco, catalog no.: 10010023) and mounted the coverslips on microscope slides using ProLong Gold Antifade Reagent (Thermo Fisher, catalog no.: P36930 ).

    Immunolabeling:

    Article Title: Sudden death-associated KCNH2 variants exert opposing effects on a nuclear subdomain of the cardiac potassium channel hERG1.
    Article Snippet: We washed the fixed cells 3 times using PBS (Gibco, Cat. No. 10010023) and stained cell nuclei using 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher, Cat. No. 62248) for 15 minutes. .. For un-transfected cells, prior to DAPI staining, we immunolabeled with a 1:150 dilution of pan-hERG primary antibody (Enzo Life Sciences, Cat. No. ALX-215049-R100) and a 1:250 dilution of goat anti-rabbit secondary antibody AF647 (Southern Biotech, Cat. No. 4050-31) to stain for the hERG1a C-terminal domain. .. We then washed 3 more times with PBS (Gibco, Cat. No. 10010023) and mounted the coverslips Jo urn al Pr -pr oo f 9 on microscope slides using ProLongTM Gold antifade reagent (Thermo Fisher, Cat. No. P36930).

    Article Title: Sudden Death-Associated KCNH2 Variants Have Opposing Effects on hERG1 NP Function
    Article Snippet: We washed the fixed cells 3 times using PBS (Gibco, Cat. No. 10010023) and stained cell nuclei using 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher, Cat. No. 62248) for 15 minutes. .. For un-transfected cells, prior to DAPI staining, we immunolabeled with a 1:150 dilution of pan-hERG primary antibody (Enzo Life Sciences, Cat. No. ALX-215-049-R100) and a 1:250 dilution of goat anti-rabbit secondary antibody AF647 (Southern Biotech, Cat. No. 4050-31) to stain for the hERG1a C-terminal domain. .. We then washed 3 more times with PBS (Gibco, Cat. No. 10010023) and mounted the coverslips on microscope slides using ProLongTM Gold antifade reagent (Thermo Fisher, Cat. No. P36930).

    Article Title: Sudden death–associated KCNH2 variants exert opposing effects on a nuclear subdomain of the cardiac potassium channel hERG1
    Article Snippet: .. After 48 h, we fixed cells with 4% paraformaldehyde in PBS for 15 min. We washed the fixed cells three times using PBS (Gibco, catalog no.: 10010023) and stained cell nuclei using 1 μg/ml 4′,6-diamidino-2-phenylindole (Thermo Fisher, catalog no.: 62248) for 15 min. For untransfected cells, prior to 4′,6-diamidino-2-phenylindole staining, we immunolabeled with a 1:150 dilution of pan-hERG primary antibody (Enzo Life Sciences, catalog no.: ALX-215-049-R100) and a 1:250 dilution of goat anti-rabbit secondary antibody AF647 (Southern Biotech, catalog no.: 4050-31) to stain for the hERG1a C-terminal domain. .. We then washed three more times with PBS (Gibco, catalog no.: 10010023) and mounted the coverslips on microscope slides using ProLong Gold Antifade Reagent (Thermo Fisher, catalog no.: P36930 ).



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